Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level.
Identifieur interne : 003A44 ( Main/Exploration ); précédent : 003A43; suivant : 003A45Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level.
Auteurs : Warren Albertin [France] ; Aurélie Panfili [France] ; Cécile Miot-Sertier [France] ; Aurélie Goulielmakis [France] ; Adline Delcamp [France] ; Franck Salin [France] ; Aline Lonvaud-Funel [France] ; Chris Curtin [Australie] ; Isabelle Masneuf-Pomarede [France]Source :
- Food microbiology [ 1095-9998 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Contamination des aliments.
- génétique : Brettanomyces.
- isolement et purification : Brettanomyces.
- Boissons alcoolisées, Brettanomyces, Génotype, Répétitions microsatellites, Techniques de typage mycologique.
English descriptors
- KwdEn :
- MESH :
- analysis : Food Contamination.
- classification : Brettanomyces.
- genetics : Brettanomyces.
- isolation & purification : Brettanomyces.
- methods : Mycological Typing Techniques.
- Alcoholic Beverages, Genotype, Microsatellite Repeats.
Abstract
Although many yeasts are useful for food production and beverage, some species may cause spoilage with important economic loss. This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.
DOI: 10.1016/j.fm.2014.03.012
PubMed: 24929736
Affiliations:
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SA</wicri:regionArea><wicri:noRegion>SA</wicri:noRegion></affiliation></author><author><name sortKey="Masneuf Pomarede, Isabelle" sort="Masneuf Pomarede, Isabelle" uniqKey="Masneuf Pomarede I" first="Isabelle" last="Masneuf-Pomarede">Isabelle Masneuf-Pomarede</name><affiliation wicri:level="1"><nlm:affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France; Bordeaux Sciences Agro, F-33175 Gradignan, France. 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This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Cestas</li><li>Gradignan</li><li>Villenave d'Ornon</li></settlement></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Albertin, Warren" sort="Albertin, Warren" uniqKey="Albertin W" first="Warren" last="Albertin">Warren Albertin</name></region><name sortKey="Delcamp, Adline" sort="Delcamp, Adline" uniqKey="Delcamp A" first="Adline" last="Delcamp">Adline Delcamp</name><name sortKey="Goulielmakis, Aurelie" sort="Goulielmakis, Aurelie" uniqKey="Goulielmakis A" first="Aurélie" last="Goulielmakis">Aurélie Goulielmakis</name><name sortKey="Lonvaud Funel, Aline" sort="Lonvaud Funel, Aline" uniqKey="Lonvaud Funel A" first="Aline" last="Lonvaud-Funel">Aline Lonvaud-Funel</name><name sortKey="Masneuf Pomarede, Isabelle" sort="Masneuf Pomarede, Isabelle" uniqKey="Masneuf Pomarede I" first="Isabelle" last="Masneuf-Pomarede">Isabelle Masneuf-Pomarede</name><name sortKey="Miot Sertier, Cecile" sort="Miot Sertier, Cecile" uniqKey="Miot Sertier C" first="Cécile" last="Miot-Sertier">Cécile Miot-Sertier</name><name sortKey="Panfili, Aurelie" sort="Panfili, Aurelie" uniqKey="Panfili A" first="Aurélie" last="Panfili">Aurélie Panfili</name><name sortKey="Salin, Franck" sort="Salin, Franck" uniqKey="Salin F" first="Franck" last="Salin">Franck Salin</name></country><country name="Australie"><noRegion><name sortKey="Curtin, Chris" sort="Curtin, Chris" uniqKey="Curtin C" first="Chris" last="Curtin">Chris Curtin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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